Human diagnostic Mycoplasma susceptibility testing kits
midstream urine or secretion
by sea/air is available
100000 Box/Boxes per Day OEM
Packaging & Delivery
Micoplasma susceptibility testing kits
Encode standard ; 20 Test / Kit , indiviudal foil package ; OEM is available .
Human diagnostic Micoplasma testing kits/Encode Micoplasma rapid kit
1.Isolation, identification, enumeration and antibiotic susceptibility tests of UU and MH. 2.High sensitivity and specificity
4. Human diagnostic Mycoplasma UU-MH testing kits
For the isolation, identification, enumeration and antibiotic susceptibility tests of urogenital tract
Ureaplasma Urealyticum (UU) and Mycoplasma Hominis (MH) in one step.
The test kit consists of freeze-dried type culture broth and susceptibility test boards. The broth contains peptone, yeast extract, serum, growth factors and other nutrients, and urea, arginine as well. The alkaline material produced by urea, arginine decomposition causes the broth pH value rise, so the broth color changes from yellow to red. The broth has antibacterial agent which inhibits the growth of bacteria and fungi. The test board’s wells are used for control test and the UU and MH identification, enumeration and susceptibility tests.
1) Urea-arginine broth (freeze-dried): 20 vials
2) Susceptibility test board: 20 pcs
3) Sterile pipette tip: 20 pcs
4) Sterile paraffin oil: 2 bottles
5) Sterile diluting liquid: 2 bottles
6) Instruction: 1 pcs
7) Report booklet: 1 pcs
STORAGE AND PERIOD OF VALIDITY
Stored at 2-8°C ; 12 months. SUSCEPTIBILITY FOR TEST
Male: Urethral secretions, prostatic fluid, semen or urine sediment (Urine 10ml centrifugated at 2000 rpm for 10 minutes);
Female: Secretion from cervix opening or the amniotic fluid collected with vaginal speculum.
Or: 30-50μl freshly cultured positive broth.
SUCCESSIVE OPERATING PROCEDURES
1) Take one urea-arginine broth (freeze-dried) vial, add sterile diluent to the scale line (3.5ml), and shake well;
2) Negative control test. Take one test board, draw 100μl diluted broth (no including any sample) with sterile pipette into A1 negative well;
3) Sample inoculation. Immerce the sample swab into the broth, stir and mix with the broth thoroughly, discard cotton swabs. For the positive cultured broth, just add 30-50μl into the broth vial. After adding samples, fully mix with shake;
4) Add 100μl suspension to the rest wells;
5) Sealing wells. Add 1-2 drops aseptic paraffin oil to all wells (Note: Be sure to fully cover each well's broth surface. Otherwise broth evaporation causes incorrect results.);
6) Culture. Cover and put the test board in an incubator, culture at 35-37°C and observe the results
within 24 or 48 hours.
1) Identification and enumeration: Observe and keep the result of Ureaplasma Urealyticum (UU) after
24 hours; Observe and keep the result of Mycoplasma Hominis (MH) after 48 hours. The results are shown for example:
2) Antibiotic Susceptibility: Wells of line A contains antibiotics in lower concentration, while line B
higher. Wells A to B, one by one contains the same antibiotic. The results are shown for example:
1) Wells show no color change or color slightly change. The samples contain ver y low titers of
2) Sample's antibiotic sensitivity test result by this kit does not match its real value: The concentration of the suspension may not be at the ideal level;
3) The broth clarity is low when reading the results. The broth keeps transparent and clear when Mycoplasma grows. In case of bacterial contamination, the broth may be muddy or red. This should not be reported Mycoplasma-positive (But some samples inoculated with more secretion into the broth may bring the color change, please note the difference);
4) Susceptibility test result(s) is unreasonable, e.g.: High concentration well turns red while low concentration one not. This case may be caused by sample's uneven concertration. This result does not make sense, please repeat the test or ignore the antibiotic's susceptibility test results.
Ideal test results should fully comply with the above testing procedures. Because all of the microbial test results largely depend on the sample collection, and if sample collection is not correct, it will lead to erroneous test results. So one negative test result does not definitely mean no Mycoplasma infection. On the other hand, clear and transparent color changes (yellow changes to red) only illustrates the Mycoplasma exist, however, the result can only be a reference to clinical diagnosis. If necessary, other methods or equipment are needed. Professionals should consult the further test results and clinical symptoms to make judgments.